Osteoarthritis Affected Reversal of Chondrocyte Senescence, Published in Scientific Reports
TOKYO – The cartilage of the knee joint, when severely damaged by wear and tear or age-related injuries or osteoarthritis, which is removed and usually discarded during joint replacement surgery, could now be rejuvenated, becoming into a potential source of cells for cell therapy through a disruptive technology that reverses aging, Confirmed by Ss-galactosidase associated with senescence, p16 and p21, markers, published in Scientific reports ( www.nature.com/articles/s41598-021-93607-9), a journal Nature. A conversation between the (late) Prof. Masatoshi Koshiba, (Nobel Laureate, 2002) and Dr. Masahiro Katoh about whether his aging joints could be rejuvenated, inspired orthopedist Dr. Shojiro Katoh to develop a path that broke the road. EELS-TALC technique (enriched with Essentials and scaffold-lapped, transplantable leveraged autologous chondrocytes), which makes this achievement.
Reversal of aging of human tissue in the laboratory, with a revolutionary tissue engineering method: EELS-TALC, opens the way to many regenerative medicine solutions; SA-B-Gal markers, p16 and p21 down regulation published in Scientific Reports, a nature journal
The aging of the human body, its organs, tissues and cells is irreversible and in laboratory settings, aging is much faster than within the body. However, the EELS-TALC technology has disrupted this conventional belief by rejuvenate tissues through tissue engineering on a chemically synthesized polymer scaffold, under a physical maneuver of orbital shaking in the laboratory, without genetic manipulation or animal derived products, Thus paving the way towards solutions that, when a similar environment is available within the body, such a reversal of aging could be possible.
EELS TALC cultured chondrocytes have all the ideal characteristics, worthy of being recommended as the most suitable in ACI and MACI treatments:
(i) yield Hyaline cartilage native to articular joints:
(ii) Hyaluronic acid enrichment, ideal for better healing:
(iii) Enhanced MiRNA 140, a distinctive mark of healthy cartilage:
(iv) Chondroprogenitors, mesenchymal stem cells grown from damaged tissues:
(v) Pluripotency marker expression and tissue as growth:
(saw) Increased length of telomeres. showing youth:
Presented at meetings of the British Society for Developmental Biology – Genetics Society and British Gerontology Society; 2021.
(vii) SA-ß-Gal lower, p16, p21; confirming the reversal of aging:
(viii) Preclinical studies that prove, in vivo success:
The EELS-TALC method is safe as it does not involve genomic alterations, oncogenes, viruses or complex growth factors. Now, Dr. Katoh is evaluating the ideal transport of tissues and cells between the hospital and the laboratory based on a previous report. OPTRACT method ( https://doi.org/10.1007/s10529-021-03116-y) and additional safety parameters, endorsed by EELS, JBM Inc, GN Corporation and NCRM, India, prior to commencing clinical studies.
Samuel JK Abraham